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FluoroVette

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Ultra-low Volume Bio Micro Cuvette for Fluorescence Detection is available in two formats:
– pipette tip interface, for standard 20-200 microliter pipetter or
– continuous flow cell with hose barb connections for standard peristaltic or syringe pump

FluoroVette™ Pipette Interface

fluorescence-detection

Fluorescence Detection with UV excitation to 220nm, 15 microliter volume

FluoroVette™ Flowcell Format

continuous-monitoring

Continuous monitoring for post-column fluorescence detection with standard fluorometer

Some of the benefits for laboratories are listed below

table-fluorovette

For the cost of 1 quartz cuvette you can have 40 FluoroVettes™
40-fluorovettes

Click here for a printable order form

Quantitation of dsDNA Using The 15 Microliter Volume,Re-usable and displosable FluoroVette

ALine’s FluoroVette™ (patent pending) is used for applications involving fluorescence detection. It fits into all standard fluorometers using ALine’s Universal Adapter. The design of the FluoroVette™makes it suitable for a variety of applications, including quantitation of double-stranded DNA (dsDNA). This application note details the use of the FluoroVette™ in conjunction with the fluorescent nucleic acid stain PicoGreen® for the quantitation of dsDNA in the 100-20,000pg range. DNA Quantitation using the FluoroVette (PDF:100K)

Dose-Response Curve
Using FluoroVette With
A Higher Concentration Series

response-curve-2

Dose-Response Curve
Using FluoroVette With
A Lower Concentration Series

response-curve-1

Conclusion

The FluoroVette™ flowcell is a convenient, sensitive, precise, and disposable companion to the PicoGreen® assay reagent in the high sensitivity quantitation of dsDNA. Its universal compatibility with all types of fluorometers, highlights its adaptability to all potential applications involving fluorescent detection.

Click here for a printable order form

Path Length .75 mm
Window UV transparent COP
Support Black Delrin
Pippette Interface Acrylic
Body Black Delrin
Bonding Biocompatible Acrylic
STANDARD PIPETTE INTERFACE
Part Number FP010 FPA10 FP020 FPA20
Unit of Sale 10 10 + adaptor 20 20 + adaptor
Price $125 $150 $150 $200
FLOWCELL CONFIGURATION
Tubing barbs use 1mm ID silicone tubing
Part Number FL005 FLA05 FL010 FL050
Unit of Sale 5 5 + adaptor 10 50 + adaptor
Price $100 $125 $165 $650
UNIVERSAL FLUOROVETTE ADAPTOR SPECIFICATIONS
PART NUMBER AD015-360
Body Material Black ABS
FluoroVette orientation 45 degrees
Z-height 15 mm
Price $60.00
How to Fill a Fluorovette?

The pipette interface FluoroVette can be conveniently filled with a standard pipetter using ordinary tips. The attached pdf gives step by step visual instructions. How to fill a FluoroVette (PDF:106K)

What tubing do I use for the Hose Barb connections?

Any standard soft tubing with an inner diameter (ID) between 1mm to 1.5 mm will work. Pharmed, silicone, and tygon tubing have all been successfully used.

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