Ultra-low Volume Bio Micro Cuvette for Fluorescence Detection is available in two formats:
– pipette tip interface, for standard 20-200 microliter pipetter or
– continuous flow cell with hose barb connections for standard peristaltic or syringe pump
FluoroVette™ Pipette Interface
Fluorescence Detection with UV excitation to 220nm, 15 microliter volume
FluoroVette™ Flowcell Format
Continuous monitoring for post-column fluorescence detection with standard fluorometer
Some of the benefits for laboratories are listed below
For the cost of 1 quartz cuvette you can have 40 FluoroVettes™
Quantitation of dsDNA Using The 15 Microliter Volume,Re-usable and displosable FluoroVette
ALine’s FluoroVette™ (patent pending) is used for applications involving fluorescence detection. It fits into all standard fluorometers using ALine’s Universal Adapter. The design of the FluoroVette™makes it suitable for a variety of applications, including quantitation of double-stranded DNA (dsDNA). This application note details the use of the FluoroVette™ in conjunction with the fluorescent nucleic acid stain PicoGreen® for the quantitation of dsDNA in the 100-20,000pg range. DNA Quantitation using the FluoroVette (PDF:100K)
Using FluoroVette With
A Higher Concentration Series
Using FluoroVette With
A Lower Concentration Series
The FluoroVette™ flowcell is a convenient, sensitive, precise, and disposable companion to the PicoGreen® assay reagent in the high sensitivity quantitation of dsDNA. Its universal compatibility with all types of fluorometers, highlights its adaptability to all potential applications involving fluorescent detection.
|Path Length||.75 mm|
|Window||UV transparent COP|
|STANDARD PIPETTE INTERFACE|
|Unit of Sale||10||10 + adaptor||20||20 + adaptor|
|Tubing barbs use 1mm ID silicone tubing|
|Unit of Sale||5||5 + adaptor||10||50 + adaptor|
|UNIVERSAL FLUOROVETTE ADAPTOR SPECIFICATIONS|
|Body Material||Black ABS|
|FluoroVette orientation||45 degrees|
How to Fill a Fluorovette?
The pipette interface FluoroVette can be conveniently filled with a standard pipetter using ordinary tips. The attached pdf gives step by step visual instructions. How to fill a FluoroVette (PDF:106K)
What tubing do I use for the Hose Barb connections?
Any standard soft tubing with an inner diameter (ID) between 1mm to 1.5 mm will work. Pharmed, silicone, and tygon tubing have all been successfully used.