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Quantitation of dsDNA Using The 50 Microliter Volume,
Re-usable and Disposable FluoroVette


ALine’s FluoroVette™ (patent pending) is used for applications involving fluorescence detection. It fits into all standard fluorometers using ALine’s Universal Adapter. The design of the FluoroVette™makes it suitable for a variety of applications, including quantitation of double-stranded DNA (dsDNA). This application note details the use of the FluoroVette™ in conjunction with the fluorescent nucleic acid stain PicoGreen® for the quantitation of dsDNA in the 100-20,000pg range. DNA Quantitation using the FluoroVette (PDF:100K)


 

Dose-Response Curve Using FluoroVette
With A Higher Concentration Series


Dose-Response Curve Using FluoroVette
With A Lower Concentration Series


 
 
high concentration dose curve
Low concentration dose curve
 

Conclusion

The FluoroVette™ flowcell is a convenient, sensitive, precise, and disposable companion to the PicoGreen® assay reagent in the high sensitivity quantitation of dsDNA. Its universal compatibility with all types of fluorometers, highlights its adaptability to all potential applications involving fluorescent detection.

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