ALine, Inc. – Accelerated Microfluidic Development


Breadboard Instrumentation

Visit us at booth #4 at LOAC WC in San Diego, Sept. 18th & 19th.

One of the more complex challenges in developing a Lab on a Chip product is integrating the instrument with the microfluidic chip.

Along with our ability to rapidly prototype microfluidic devices, moving quickly through the design-build-test cycle, we offer mechanical, electrical, and software engineering to integrate the microfluidic into a small stand alone system as described in our instrumentation capabilities brochure.

For a preview of our capabilities, see our September Newsletter

Newsletter August 2014

View our August Newsletter

- Learning Lab at Micro-TAS October 29th, 2014

- ALine’s sealing and lidding capabilities

- ALine’s partners for on-Chip reagent storage

ChroMedX Engages ALine to Develop the HemoPalm

July 29, 2014 – Toronto, Ontario – Monarch Energy Limited (CSE: CHX) (OTC: MNLIF)(“Monarch” or the “Company“) is pleased to announce that its wholly-owned subsidiary ChroMedX Ltd. has engaged ALine Inc. of Rancho Dominguez, CA as its primary contractor for the development of the Company’s HemoPalm cartridge and analyzer technology. The goal is to complete construction of the prototype cartridge and analyzer to demonstrate functionality of the system by the first quarter of 2015. ALine has expertise in the engineering, prototyping and manufacturing of microfluidic cartridges used in the life sciences and in vitro diagnostic fields. ALine Inc. was founded by current President and CEO, Leanna Levine, Ph.D.


Wayne Maddever, Ph.D. P.Eng, President and CEO of ChroMedX Ltd. said, “The experience and expertise of Leanna and her ALine team make them the perfect partner for the development of the HemoPalm system.”


Leanna Levine, Ph.D., President and CEO of ALine said, “We are excited about the potential for ChroMedX’s technologies and are pleased to be a part of this development.”


HemoPalm Technology

The HemoPalm system is comprised of a disposable cartridge and a handheld analyzer. The system measures co-oximetry, blood gases and electrolytes, in a patient’s blood, and the resulting analysis can provide a complete assessment of a patient’s acid-base and oxygenation status at the point of care (POC). The leading competitors in POC testing require a second analyzer to provide CO-oximetry, which is the gold standard for measuring hemoglobin oxygen saturation and total hemoglobin. The ability to provide all the measurements described above in a single central laboratory analyzer has been available since the 1990s. ChroMedX intends to be the first company to provide these measurements in a single handheld analyzer.

In addition to ALine Inc., ChroMedX has engaged additional consultants with expertise in microspectrometry and biosensors to compliment the development team led by James Samsoondar, Ph.D., ChroMedX Chief Scientific Officer.

Microfluidic Chip that Meters, Mixes, Debubbles, and Dispenses – M2D2

June 10, 2014 ALine’s tool box of engineered components speeds development and lowers risk in microfludic chip development. This was the theme of a talk presented at the Biodetection Technologies Conference in Baltimore, MD. For applications where low risk, robust performance and reliability are a must, our on-board pneumatically actuated valves and pumps are well-characterized and have been used for nearly ten years for Lab-on-a-Chip applications. The microfluidic chip, the M2D2, meters, mixes, debubbles, and dispenses with control from either an 8 or 12 channel pneumatic instrument, the ADEPT, a microfluidic testing and development platform.

ALine Becomes Member of NSF Center for Advanced Design and Microfluidics Manufacture

January 31, 2014 – ALine will support programs at UC-Irvine, and U Cincinnati aimed at advancing microfludics manufacture for Health and Life Sciences applications. ALine is pleased to participate as a technical advisor along with industry partners and faculty members of the NSF center. The goal is to develop novel applications of interest to CADMIM’s industrial partners with a focus on manufacturing technologies.

Microfluidic Cell Culture Device Patent Awarded

November 15, 2013 – After a seven year process with the USPTO, a patent has been awarded for our controlled flow microfluidic cell culture device (the MicroSlide). This cell culture card was developed for the NASA Astrobionics program in 2004-2005. The unique advantages of this microfluidic cell culture card are: 1) a single inlet channel feeds each of the cell culture chambers at the same rate; 2) sterile isolation of the contents of each chamber.


The principle of the MicroSlide’s unique flow control relies on the placement of macroporous membranes as the inlet and outlet of each fluid chamber. The membrane area, porosity, and thickness contribute to the resistance of fluid flow into and out of each chamber. As a result only a single inlet channel and outlet channel are required to maintain equivalent flow across each cell culture chamber.

Contact us to learn more how this product can be used for your cell culture experiments.

The NASA Astrobionics program’s mission is to conduct biological experiments in near earth orbit on autonomous satellites engineered to grow and maintain living cell cultures. The goal is to study the effect of weightlessness and radiation on growing cells. The stringent requirements for compact size, low power consumption, and ruggedness resulted in the successful launch an automated satellite system the size of a loaf of bread (nano satellite) that had ALine’s controlled flow microfluidic cell culture card in the heart of the system.

To learn more about the cell culture card’s flight into near earth orbit, see our case studies. Or visit our technical articles page to read the publication.

New Website to Launch on September 15th

Our new website will describe our services more thoroughly, and include a section on case studies of successful programs we have worked on.

On Board Metering, Mixing, Debubbling and Dispensing

Our video demonstrates four key functions for multi-step tests in a microfluidic device: Metering, Mixing, Degassing before Dispensing to the detection region.We demonstrate metering of two different volumes to create a 1:10 dilution, mix, debubble and then deliver to a detection region for imaging.

The chip shown in the video we used multiple times and performed all the steps completely, even with repeated use.

Looking to the Future

It was nice to connect with current customers, and to get the word out about our capabilities at MD&M West. We find more and more new biotech start ups in the LA basin that are glad ALine is close by. This event for us is like a party where we can socialize and learn what’s new with our business partners and customers. Doing something new is what keeps every business current and able to adapt to shifts in customer needs. This year we are focusing on automation development to support mid-volume production of fluid circuit devices. We are expanding our offering to include design and prototyping of injection molded components, as well as thermal and direct bonding of polymer films. We are investing in more robust blister pack technology for prototyping and low volume production along with reagent deposition capabilities. This will allow our customers to integrate and source all the components needed for a complete product solution.

Visit us at MD&M West Feb 12-14 Booth 1384

Join us at this year’s MD&M West. Our pneumatic instrument platform now has remote control using either a smart phone or iPad. Talk with our engineering staff to learn how we can support your product development program. to learn more about our automation capability, see our January  NewsletterALine MD&M West Booth 1384