ALine, Inc. – Accelerated Microfluidic Development


3D Printed Microfluidic Connectors Solve the Chip-to-World Interface Problem

3D printing lends itself to the manufacture of components which are tailored to specific needs of the end user. For example, an early application of 3D printers was in the manufacture of custom hearing aid shells, designed to fit comfortably into the ear of the individual patient. In dentistry, it has been used to create crowns, mouth guards and braces. Applications in the Life Sciences and Medical Device industry continue to grow as the print resolution, and available materials expand. Exotic applications in tissue engineering, with the goal of producing organs and tissues with defined composition, are being explored.

For applications in microfluidics, ALine has developed a series of 3D printed fluidic connectors that integrate with custom microfluidic devices produced with rapid prototyping techniques such as lamination, or micro-machining. By comparison, commercial connectors, such as luer-locks and hose barbs, have only a limited choice in terms of geometry, require a large footprint for multiple inputs, and introduce dead volume into the fluidic network. Some tubing connectors with compression fittings are available with low dead volume, but are priced so high they often cost more than the device itself.

3D printed fluidic connectors greatly expand the options available to bridge the “chip-to-world” interface. The micro-to-macro interface between the microfluidic chip and the supporting instrumentation has always been a difficult engineering problem. This interconnection issue sometimes leads to the assertion that microfluidics really represents a “Chip in a Lab” rather than a “Lab on a Chip”.

With the development of low cost, high resolution 3D printers, the interface problem can be overcome with simple, easily customized connections. 3D printed connectors with multiple inputs occupy a much smaller footprint and minimize the dead volume. Connectors are customized for the specific device configuration to allow 90 degree of co-planar connections. Hose barbs for flexible tubing connections, as shown in Figure 1) and 2), are a typical application and are readily assembled to the microfluidic using biocompatible pressure sensitive adhesives.

3D printed connector integrated into device

Figure 2) Low Dead Volume Connection Co-planar with the Microfluidic Device

The process to produce these connectors is suitable for rapid prototyping and low to mid-volume manufacturing. The turn-around time is typically a few hours because the process does not involve any machining or production of molds. 3D designs are created using computer automated design (CAD)

3D connector

Figure 1) 3D Printed Multiplexed Tube Connection

software. The printer selectively cures 2D layers of a UV resins, building successive layers which produce 3D objects. Multiple resins are available allowing for choice of color, mechanical properties and biocompatibility. Typical batches include 10-20 connectors per 3D print run with scale-up to 100s and even 1000s easily achieved with no change in equipment.

ALine’s approach of combining 3D printed connectors with our proprietary laminate fabrication technology provides design flexibility in the development of custom microfluidic devices. With the advanced 3D printing tools now available, we solve the problem of the “Chip-to-World” interface with customized connectors, tailored to meet the unique design and functional requirements of each microfluidic application.

For more information contact:  Stefano Begolo, Ph.D.;  sbegolo(at)

ALine Is Growing!

We are pleased to announce that Dr. Stefano Begolo, a recent post doctoral fellow in Rustem Ismagilov’s lab, is joining ALine, in early August 2015.

Stefano was a customer while he was working on the Slip Chip technology at CalTech and he enjoyed interacting with us enough to be interested in being part of the team!

Stefano’s expertise in microfluidics, his familiarity with assay development and PCR, and his interest in working on a variety of client programs will add tremendous depth to our offering.

With his expertise in 3D printing, we plan to offer this capability along with our popular laminate technology.

Click here to learn more about Stefano.

Integrated & Automated Fluid Actuation

The ADEPT Automates Potentiometric Measurements Using a Microfluidic card connected to an Ion Selective Electrode (ISE) Sensor Array

We combined a low noise multichannel electrometer with the ADEPT pneumatic control instrument for sequential testing of an ISE sensor array to measure Na+, K+, and pH using an external reference. The test system includes an 8-channel ADEPT instrument with the electrometer housed in a shielded box. A slide in port for the microfluidic test card reduce ambient noise and the automated routine for delivering three different analyte solutions of Na+, K+, and pH, provided a controlled environment for the ISE measurements.June Newsletter Figure 1

Read our latest Newsletter to learn more.

George Lauro, Distinguished Tech Entrepreneur, Joins ALine’s Board

6 x 96 valve manifold

ALine’s Advanced 6 x 96 Valve Manifold

ALine, a leading developer of advanced Fluid Circuit products and solutions is pleased to announce that George Lauro has joined its board. George has 25 years of experience as a technology entrepreneur, operating executive, and venture capitalist. He has built several tech companies from early stage to high value exit (M&A or IPO). George is Founding Partner of Alteon Capital, a Technology Venture Development firm based in Cambridge, MA. He was Managing Director at Wasserstein Perella and head of West Coast technology investing, and was a Partner at Techfarm Venture Capital in Silicon Valley. He led and syndicated 18 private equity financing rounds and control deals, raised over $100M equity financing for portfolio companies and completed over $1 billion in M&A transactions. George will work with ALine’s management team to develop and execute its accelerated growth strategy.

George Lauro added, “I am delighted to be joining ALine’s Board. I believe that the Company’s proprietary functional and material set and strong design and process team positions it for significant growth in the microfluidics and Lab-on-Chip sector. I look forward to helping the Company and team achieve this potential.”

Dr. Leanna Levine, President and CEO commented, “We are very happy that George recognizes the growth potential for our business. His distinguished career and experience in venture capital, operations and entrepreneurship are a tremendous asset to ALine. We are pleased he recognizes ALine’s unique and valuable position in the microfluidics market space.”

About ALine
ALine is a leading microfluidic and Lab-on a Chip engineering and manufacturing company serving the Life Science, Medical Diagnostics, and Analytical test markets. Over the past 7 years, we’ve designed and brought to volume production several advanced microfluidic solutions for major customers in several industry sectors, including NASA, JPL, Abbott, GenMark, and Iris Diagnostics (Beckman Coulter).

ALine’s array of materials and fabrication processes give us the broadest design space of any Microfluidics company in the world. We provide prototype-to-production solutions and execute all steps of microfluidic solution development from initial design through prototyping up to high volume manufacturing. Our unique processes enable one week turnaround on prototypes of sophisticated microfluidic and Lab-on-Chip devices that are scalable for volume production.

ALine was founded in 2003, and is ISO9001:2008 certified.

Breadboard Instrumentation

Visit us at booth #4 at LOAC WC in San Diego, Sept. 18th & 19th.

One of the more complex challenges in developing a Lab on a Chip product is integrating the instrument with the microfluidic chip.

Along with our ability to rapidly prototype microfluidic devices, moving quickly through the design-build-test cycle, we offer mechanical, electrical, and software engineering to integrate the microfluidic into a small stand alone system as described in our instrumentation capabilities brochure.

For a preview of our capabilities, see our September Newsletter

Newsletter August 2014

View our August Newsletter

– Learning Lab at Micro-TAS October 29th, 2014

– ALine’s sealing and lidding capabilities

– ALine’s partners for on-Chip reagent storage

ChroMedX Engages ALine to Develop the HemoPalm

July 29, 2014 – Toronto, Ontario – Monarch Energy Limited (CSE: CHX) (OTC: MNLIF)(“Monarch” or the “Company“) is pleased to announce that its wholly-owned subsidiary ChroMedX Ltd. has engaged ALine Inc. of Rancho Dominguez, CA as its primary contractor for the development of the Company’s HemoPalm cartridge and analyzer technology. The goal is to complete construction of the prototype cartridge and analyzer to demonstrate functionality of the system by the first quarter of 2015. ALine has expertise in the engineering, prototyping and manufacturing of microfluidic cartridges used in the life sciences and in vitro diagnostic fields. ALine Inc. was founded by current President and CEO, Leanna Levine, Ph.D.


Wayne Maddever, Ph.D. P.Eng, President and CEO of ChroMedX Ltd. said, “The experience and expertise of Leanna and her ALine team make them the perfect partner for the development of the HemoPalm system.”


Leanna Levine, Ph.D., President and CEO of ALine said, “We are excited about the potential for ChroMedX’s technologies and are pleased to be a part of this development.”


HemoPalm Technology

The HemoPalm system is comprised of a disposable cartridge and a handheld analyzer. The system measures co-oximetry, blood gases and electrolytes, in a patient’s blood, and the resulting analysis can provide a complete assessment of a patient’s acid-base and oxygenation status at the point of care (POC). The leading competitors in POC testing require a second analyzer to provide CO-oximetry, which is the gold standard for measuring hemoglobin oxygen saturation and total hemoglobin. The ability to provide all the measurements described above in a single central laboratory analyzer has been available since the 1990s. ChroMedX intends to be the first company to provide these measurements in a single handheld analyzer.

In addition to ALine Inc., ChroMedX has engaged additional consultants with expertise in microspectrometry and biosensors to compliment the development team led by James Samsoondar, Ph.D., ChroMedX Chief Scientific Officer.

Microfluidic Chip that Meters, Mixes, Debubbles, and Dispenses – M2D2

June 10, 2014 ALine’s tool box of engineered components speeds development and lowers risk in microfludic chip development. This was the theme of a talk presented at the Biodetection Technologies Conference in Baltimore, MD. For applications where low risk, robust performance and reliability are a must, our on-board pneumatically actuated valves and pumps are well-characterized and have been used for nearly ten years for Lab-on-a-Chip applications. The microfluidic chip, the M2D2, meters, mixes, debubbles, and dispenses with control from either an 8 or 12 channel pneumatic instrument, the ADEPT, a microfluidic testing and development platform.

ALine Becomes Member of NSF Center for Advanced Design and Microfluidics Manufacture

January 31, 2014 – ALine will support programs at UC-Irvine, and U Cincinnati aimed at advancing microfludics manufacture for Health and Life Sciences applications. ALine is pleased to participate as a technical advisor along with industry partners and faculty members of the NSF center. The goal is to develop novel applications of interest to CADMIM’s industrial partners with a focus on manufacturing technologies.

Microfluidic Cell Culture Device Patent Awarded

November 15, 2013 – After a seven year process with the USPTO, a patent has been awarded for our controlled flow microfluidic cell culture device (the MicroSlide). This cell culture card was developed for the NASA Astrobionics program in 2004-2005. The unique advantages of this microfluidic cell culture card are: 1) a single inlet channel feeds each of the cell culture chambers at the same rate; 2) sterile isolation of the contents of each chamber.


The principle of the MicroSlide’s unique flow control relies on the placement of macroporous membranes as the inlet and outlet of each fluid chamber. The membrane area, porosity, and thickness contribute to the resistance of fluid flow into and out of each chamber. As a result only a single inlet channel and outlet channel are required to maintain equivalent flow across each cell culture chamber.

Contact us to learn more how this product can be used for your cell culture experiments.

The NASA Astrobionics program’s mission is to conduct biological experiments in near earth orbit on autonomous satellites engineered to grow and maintain living cell cultures. The goal is to study the effect of weightlessness and radiation on growing cells. The stringent requirements for compact size, low power consumption, and ruggedness resulted in the successful launch an automated satellite system the size of a loaf of bread (nano satellite) that had ALine’s controlled flow microfluidic cell culture card in the heart of the system.

To learn more about the cell culture card’s flight into near earth orbit, see our case studies. Or visit our technical articles page to read the publication.